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KMID : 0861020230380010001
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Korea Journal of Herbology
2023 Volume.38 No. 1 p.1 ~ p.9
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Effects of Baicalein on hydrogen peroxide productions in RAW 264.7 mouse macrophages stimulated by peptidoglycan
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Park Wan-Su
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Abstract
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Objectives : The aim of this study was to investigate the effect of baicalein (BA) on the production of hydrogen peroxide in peptidoglycan-stimulated RAW 264.7 mouse macrophages.
Methods : Peptidoglycan-stimulated RAW 264.7 were incubated with baicalein at concentrations of 50 and 100 μM. Incubation time is 30 min, 2 h, 12 h, and 18 h. After incubation, The production of hydrogen peroxide in RAW 264.7 was measured with dihydrorhodamine 123 assay. Berberine and gallic acid were used as the comparative materials.
Results : BA at the concentration of 50 and 100 μM did not show cytotoxicity on RAW 264.7 for 24 h incubation. For 30 min, 2 h, 12 h, and 18 h incubation, BA at the concentration of 50 and 100 μM significantly inhibited the production of hydrogen peroxide in RAW 264.7 stimulated by peptidoglycan (p < 0.05). In details, production of hydrogen peroxide in peptidoglycan-stimulated RAW 264.7 treated for 30 min with BA at concentrations of 50 and 100 μM was 93.91% and 93.52% of the control group treated with peptidoglycan only, respectively; the production of hydrogen peroxide for 2 h was 93.8% and 92.71%, respectively; production of hydrogen peroxide for 12 h was 94.86% and 95.93%, respectively; production of hydrogen peroxide for 18 h was 95.37% and 96.48%, respectively.
Conclusions : BA might have anti-oxidative activity related to its inhibition of hydrogen peroxide production in peptidoglycan-stimulated RAW 264.7 macrophages.
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KEYWORD
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RAW 264.7, Baicalein, Macrophage, Peptidoglycan, Hydrogen peroxide
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